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Nikon TIRF System
| TIRF is a technique used to image a thin region (less than 200nm) close to the surface of a specimen at high resolution. This is useful in cell and molecular biology to observe events occurring on cellular surfaces such as cell adhesion, binding of hormones, secretion of neurotransmitters, and membrane dynamics. On a TIRF microscope, the illumination and excitation light is directed at such an angle to the sample that it is totally internally reflected. When this happens, there is a small evanescent wave that passes through the sample and allows for the selective excitation of the fluorophores in a restricted region of the specimen immediately adjacent to the glass-water interface. The associated electromagnetic field decays exponentially from the interface, and thus penetrates to a depth of only approximately 100 nm into the sample. |
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Training Courses Available -------------------------------------------------------------------------------- Widefield Microscope Training Advanced Widefield Techniques Model -------------------------------------------------------------------------------- Nikon TE2000 Objective Lenses -------------------------------------------------------------------------------- Plan Fluor 10x /0.25 Ph1 Plan Fluor 40x /0.6 Ph2 Plan Apo 20x/0.75 Ph2 Apo TIRF 60x /1.49 oil Illumination Sources -------------------------------------------------------------------------------- Xenon 488nm Argon laser Filter Sets -------------------------------------------------------------------------------- Chroma DAPI/FITC/Cy3/Cy5 Quad Sedat (only available for standard widefield mode) GFP (TIRF mode only) Cameras -------------------------------------------------------------------------------- Hamamatsu ORCA-ER Software -------------------------------------------------------------------------------- Metamorph Advanced Advantages and Techniques -------------------------------------------------------------------------------- Perfect Focus System Prior motorized stage II Environmental Chamber |
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