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Comprehensive Course on Fluorescence Microscopy

Bob and Joan Wright Cell Imaging Facility (WCIF)

Under the leadership of Dr. Elise Stanley, the Bob and Joan Wright Cell Imaging Facility was established at the Toronto Western Hospital (now the Krembil Research Institute) in the year 2000 through a generous gift from the Wright Family. A second donation from the Wrights along with a CFI equipment grant enabled a complete renewal of the equipment in 2017, allowing the WCIF to again offer state-of-the-art optical microscopy to Krembil researchers and the community. At the same time, the Krembil partnered with the AOMF to staff and manage the facility, tapping into a proven team of microscopy experts to allow researchers to get the most benefit from the advanced instrumentation.

Here's a quick virtual tour:
  • The Zeiss LSM880 confocal microscope is fully loaded with high-sensitivity GaAsP detectors, a stage-top incubator for live-cell imaging, a motorized stage for tiling large fields-of-view, Airyscan for enhanced resolution, and more.
  • The Leica SP8 Two-Photon, which is configured for intravital imaging, has 2 regular confocal lasers as well as a Coherent Discovery femtosecond laser.
  • The fully-motorized Zeiss AxioObserver widefield microscope has a stage-top incubator and Definite Focus (hardware autofocus) for stable multi-location overnight timelapse imaging. It is also suitable for fluorescence imaging of fixed slides when confocal is not required.
  • The Zeiss AxioZoom Macroscope has a large field-of-view for gross sections or whole-organ fluorescence or brightfield imaging.
  • The MBF Stereology system applies statistical methods to systematically count features in tissue sections.
  • A convenient drop box gives Krembil researchers easy access to our Aperio AT2 (brightfield) and Zeiss Axioscan (fluorescence) slide scanners.
  • A cell-culture suite supports live-cell studies.
  • The WCIF image analysis station gives you access to the latest version of Imaris for 3D rendering and analsis.

Kevin Conway, Ph.D. The AOMF's site manager for the WCIF is Dr. Kevin Conway. Please contact Kevin for a free tour and consultation, to book trainings or support sessions, or to report problems with the microscopes.

Finding the WCIF
Krembil personnel: Take the KDT service elevators to 7th floor - the WCIF is opposite the elevators.

External visitors: Please make your way to the Second Cup at Toronto Western Hospital, then call Kevin at 647-705-2848.

Mailing Address:
60 Leonard Avenue, Room 7KD-492
Toronto, ON, M5T 0S8

Check out what our other sites have to offer:
- Princess Margaret Cancer Research Tower (PMCRT)
- Toronto General Hospital (TGH)
- Princess Margaret (PM)

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