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O-MUG

Comprehensive Course on Fluorescence Microscopy
Axiovert Training I: Introduction to the Axiovert 200M

Approximate Time: 2 hours
Pre-requisite: None
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This training session is a hands-on microscopy course designed as an introduction to the Axiovert 200M. Users will be shown how to operate every aspect of the microscope and taught the theory behind each component and how it relates to acquiring the most optimal image from your specimen. Finally, the user will capture images using a CoolSnap HQ CCD camera with Image Pro Plus. Topics covered in this course include:

1. Basic Microscopy
2. Activating Microscope Components
3. Motorized Controls
4. Transmission Light Path
5. Kohler Illumination (contrast, resolution, magnification)
6. DIC, Phase Contrast (including theory)
7. Fluorescence Light Path
8. Filter Cubes
9. Photobleaching
10. Optimizing Fluorescence Image
11. Image Acquisition with Image Pro Plus
12. Microscope Care and Maintenance
13. Shutting Down

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.
Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.