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How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy


Calcium Imaging System

This system is ideal for calcium ratiometric imaging, with automated multichannel and multi-Z plane acquisitions capabilities, microinjection, perfusion with temperature control, high NA lenses, high resolution and high speed CCD camera for fluorescence live cell imaging.
Model
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Zeiss AxioObserver Z1

Objective Lenses
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10x 0.50na Fluar with DIC optics
40x 1.30na oil Fluar with DIC optics

Illumination Sources
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Sutter DG4 Fluorescence illumination system

Filter Sets
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DAPI(350ex/460em), GFP/FITC (470ex/525em), Texas Red (560ex/630em) filter sets

Cameras
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Hamamatsu ORCA AG High Resolution 1344x1024 CCD camera

Software
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Volocity (Perkin Elmer)

Other components
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- Burleigh PCS-6000 micromanipulators (left and right)
- Rolera XR
- Warner V6 six channel valve controller
- Harvard TC-202A temperature controller
- Faraday cage enclosure
- High Performance Acquisition computer workstation

For more information on CBTC and equipment, please visit: http://cbtc.utoronto.ca.


Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.