Home - Sitemap - Contact Us

Equipment

Axiovert Training I
Axiovert Training II
Olympus Upright Training
Spinning Disk Confocal Training
Confocal Training I

Two-Photon Training
Xenogen Training
Olympus Upright BX51 Training
Leica Stereomicroscope Training
Fundamentals of Microscopy
Live-Specimen Imaging
Image Processing I
Image Processing II
Introduction to Image J
Adobe Illustrator for Scientists
Comprehensive Course on Fluorescence Microscopy
Policies
Applications
Links
Contact Us
Booking Calendar

How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy
Confocal Training II

Approximate Time: 2 hours
Pre-requisite:Confocal Training I
__________________________________________________________________________

In addition to being an excellent microscope for fixed immunofluorescence samples, the confocal is an ideal platform for sophisticated imaging experiments, particularly in living cells. Once the user has become familiar with basic confocal operations and has acquired a minimal level of proficiency, they then have the option to study more advanced topics. These include:

  1. Live-cell Imaging
    • Heated stage and objective heater
    • Separating fluorescence emissions
      (eg. CFP/YFP/GFP/dsRed)
  2. FRAP (fluorescence recovery after photo-bleaching)
  3. Time-series imaging
  4. Region of interest selection
  5. Macros

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.
Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.