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Approximate Time: 2 hours
Pre-requisite: None
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There is much more to microscopy than meets the eye. Knowing how to properly operate and align a microscope is an invaluable skill that will ultimately make the difference between a poor and excellent image for both quantification and publication. There are also techniques such as differential interference contrast, darkfield and phase contrast that can drastically improve image contrast for certain specimens. Over the years, other components have been added to the standard upright microscope, further increase flexibility. Many microscopes today are equipped with mercury light sources and filters for fluorescence. Additionally, CCD cameras have all but replaced the standard 35mm cameras. Topics covered include:

Microscope Components Transmission Light Path Koehler Illumination Resolution and Contrast Magnification and Numerical Aperture DIC, Phase Contrast, Darkfield Fluorescence Microscopy Light Path Filter Sets - excitation, dichroic, emission Photobleaching Optimizing Fluorescence Image CCD Cameras and Image Acquisition Various Microscope Types Microscope Care and Maintenance

Please feel free to contact us if you have any questions or would like to discuss training on this microscope.