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How to get started at the AOMF

O-MUG

Comprehensive Course on Fluorescence Microscopy
Inverted Fluorescence Microscope

This microscope is ideal for fluorescence imaging of live cells, including automated multi-channel, multi-Z plane, and multi-XY experiments. It also can be used for deconvolution.
Model
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Zeiss AxioObserver Z1

Objective Lenses
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10x 0.30na Neofluar Ph1
20x 0.40na LD Neofluar Ph2 Korr
40x 0.60na LD Neofluar Ph2 Korr
40x 1.30na oil Plan-Apochromat Iris (UV) VIS-IR with DIC optics
63x 1.40na oil Plan-Apochromat with DIC optics

Illumination Sources
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EXFO X-Cite

Filter Sets
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DAPI(350ex/460em), GFP/FITC (470ex/525em), DsRed/CY3 (545ex/620em), TexasRed (560ex/630em) filter sets

Cameras
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Hamamatsu ORCA AG High Resolution 1344x1024 CCD camera

Software
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Volocity (Perkin Elmer)

Other components
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- ASI motorized XY stage
- High resolution CCD camera
- High Performance Acquisition computer workstation

For more information on CBTC and equipment, please visit: http://cbtc.utoronto.ca.

Copyright 2006 Advanced Optical Microscopy Facility. All rights reserved.